Mutant Spider 
Effects
- Isaac fires 4 tears at the same time.
- (Removed in Repentance) Tears down: (Delay * 2.1) + 3.
- (Added in Repentance) Tears fired per second * 0.42.
- (Added in Afterbirth †) This item belongs to the Spider Baby set. Collecting three items from this set will transform Isaac into a humanoid spider.
Notes
- (Removed in Repentance) Mutant Spider is a special item.
- The maximum amount of tears per volley that can be reached by combining and/or duplicating  20/20, 20/20, The Inner Eye, Mutant Spider and (Added in Afterbirth) The Inner Eye, Mutant Spider and (Added in Afterbirth) The Wiz is 16, or 20 with the  (Keeper) Keeper. The Wiz is 16, or 20 with the  (Keeper) Keeper.
Synergies
 Doctor's Remote/ Doctor's Remote/ Epic Fetus: Replaces the single missile strike with a barrage of four consecutive missiles. Epic Fetus: Replaces the single missile strike with a barrage of four consecutive missiles.
- (Added in Afterbirth / Removed in Repentance) The Inner Eye: Shoots 7 tears with no further decrease in fire rate. The Inner Eye: Shoots 7 tears with no further decrease in fire rate.
- (Added in Repentance) Shoots 5 tears with no further decrease in fire rate.
 
 Mutant Spider: Fires 8 tears with no further decrease in tear delay. Mutant Spider: Fires 8 tears with no further decrease in tear delay.- 
- (Added in Repentance) Each copy of the item adds +2 tears up to the maximum of 16.
 
 Polyphemus: Tear rate is not lowered further. Damage is increased by a flat +5 instead of normal Polyphemus boost. Polyphemus: Tear rate is not lowered further. Damage is increased by a flat +5 instead of normal Polyphemus boost.
Interactions
- (Removed in Afterbirth †)  20/20: Overridden by Mutant Spider. 20/20: Overridden by Mutant Spider.
- (Added in Afterbirth / Removed in Repentance) Shoots 6 tears.
- (Added in Repentance) Removes the fire rate penalty while reducing damage to 75%. Additional copies will add one additional tear with no further change to damage/fire rate.
 
- (Added in Afterbirth / Removed in Repentance)  (Keeper) Keeper: Fires 7 tears with significantly increased tear delay.
- (Added in Repentance) Fires 5 tears with significantly increased tear delay.
 
- (Added in Repentance)  (Tainted Keeper) Tainted Keeper: Fires 6 tears with significantly increased tear delay.
 The Ludovico Technique: The tear gains 3 orbiting tears. The Ludovico Technique: The tear gains 3 orbiting tears.
 Loki's Horns: Only occasionally fires one tear in the other three directions. Loki's Horns: Only occasionally fires one tear in the other three directions.
- (Removed in Afterbirth †)  Mom's Eye: No effect. Mom's Eye: No effect.
- (Added in Afterbirth †) Fires three shots out of each eye.
 
 Monstro's Lung: Adds 7 tears to the barrage with a significant increase in tear delay. Monstro's Lung: Adds 7 tears to the barrage with a significant increase in tear delay.
 Sad Bombs/(Added in Afterbirth †) Sad Bombs/(Added in Afterbirth †) Varicose Veins/(Added in Repentance) Varicose Veins/(Added in Repentance) It Hurts: No effect. It Hurts: No effect.
- (Added in Afterbirth) Tractor Beam: All tears are fired along the beam. Tractor Beam: All tears are fired along the beam.
- (Added in Afterbirth) The Wiz: Four shots are fired in each direction. The Wiz: Four shots are fired in each direction.